Enriched NGM Agar for 1 Liter

Component                       g/l

Agar                                             20.0

Sodium Chloride                              3.0

BactoTM Peptone                              2.5

Additions per liter:

Streptomycin Sulfate                    0.14

Nystatin                                      0.007

Cholesterol (5mg/ml in EtoH)        1.0ml

1M Calcium Chloride                    1.0ml

1M Magnesium Sulfate                 1.0ml

1M Phosphate Buffer pH 6.0         25ml

BactoTM and DifcoTM are trademarks of Difco Laboratories, Inc. subsidiary of Becton Dickenson and Company