NGM Agar with Uracil

for 1 Liter

Component                                  g/l

Agar                                              20.0

Sodium Chloride                              3.0

Bacto^TM Peptone                              2.5

Additions per liter:

Uracil (2mg/ml)                               1.0ml

Cholesterol (5mg/ml in EtoH)              1.0ml

1M Calcium Chloride                       1.0ml

1M Magnesium Sulfate                      1.0ml

1M Phosphate Buffer pH 6.0            25ml

Bacto^TM and Difco^TM are trademarks of Difco Laboratories, Inc. subsidiary of Becton Dickenson and Company