NGM Agar w/o Antimicrobials

 for 1 Liter

Component                          g/l

Agar                                              20.0

Sodium Chloride                              3.0

Bacto^TM Peptone                              2.5

Additions per liter:

Cholesterol (5mg/ml in EtoH)         1.0ml

1M Calcium Chloride                       1.0ml

1M Magnesium Sulfate                    1.0ml

1M Phosphate Buffer pH 6.0           25ml

Bacto^TM and Difco^TM are trademarks of Difco Laboratories, Inc. subsidiary of Becton Dickenson and Company