S  Medium

 for 1 Liter

Component                                      g/l

Sodium Chloride                              5.84

Cholesterol (5mg/ml in ETOH)           1ml

KH₂PO₄                                                      2.881

K₂HPO₄                                                    0.666

Additions:

1M Potassium Citrate pH 6.0            10ml

Trace Metals Solution                      10ml

1M CaCl₂                                                   3ml

1M MgSO₄                                        3ml